Technical Data
Chondroitin 4 Sulfate
A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.

Suitable for use in ELISA, RIA, Immunohistochemistry and Western Blot. Other applications have not been tested.

Recommended Dilutions:
ELISA: 1:400-1:3200
RIA: 1:50-1:6400
Western Blot: 1:1000-1:4000 (ECL)
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ul-20CBlue IceMouseMouse
Not Determined
Mouse proteoglycan
Supplied as a liquid.
Recognizes both mouse (fetal and adult) and human (fetal and adult) chondroitinase ABC digested cartilage proteoglycans. Chondroitin 4 sulfate disaccharides successfully inhibit the binding of monoclonal antibody to proteoglycans. No reaction with native proteoglycans or with proteoglycans digested with either chondroitinase AC or testicular hyaluronidase. Species Crossreactivity: bovine, sheep, canine and rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Ortega-Barria, E et al. (1999). A Toxoplasma Lectin-like Activity Specific for Sulfated Polysaccharides Is Involved in Host Cell Infection. J Biol Chem. 274:1267-1276. 2. Glant, T. et al. (1998). Critical roles of glycosaminoglycan side chains of cartilage proteoglycan (aggrecan) in antigen recognition and presentation. J. Immunology 160:3812- 3819. 3. Cs-Szabo, TG. et al. (1995). Large and small proteoglycans of osteoarthritic and rheumatoid articular cartilage. Arthritis Rheum 38:660-668. 4. Poole, C. et al. (1991). Chondrons from articular cartilage. (IV). Immunolocalization of proteoglycan epitopes in isolated canine tibial chondrons. J. Histochem. Cytochem. 39:1175-1187. 5. Mikecz, K. et al. (1987). Immunity to cartilage proteoglycans in BALB/c mice with progressive polyarthritis and ankylosing spondylitis induced by injection of human cartilage proteoglycan. Arthritis Rheum. 30:306-318.