Suitable for use in ELISA and RIA. Other applications not tested.
Chondroitinase ABC digestion prior to antibody reaction is required for antibody reactivity. A dramatic proteolytic digestion of the core protein (e.g. with papain or pronase) significantly reduces the antibody reactivity.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
| Not Determined|
|Chondroitinase ABC-digested adult human aggrecan.|
|Supplied as a liquid, no preservative added.|
|Recognizes human Chondroitin 6 Sulfate. Reacts with a wide range of cartilage proteoglycans after either chondroitinase (ABC or AC) or testicular hyaluronidase digestion. Antibody binding to epitope is successfully inhibited by disaccharides of chondroitin-6-sulfate. Recognizes the chondroitin-6-sulfate stubs (preferentially in clusters) of high density cartilage proteoglycans of different species. Does not react with native proteoglycans, dermatan sulfate, keratan sulfate or chondroitin-4-sulfate. Species Crossreactivity: chondroitin-6-sulfate stubs of all proteoglycans of all species.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. J. Biochem. (1986) 234: 31–41. 2. Arthritis Rheum. (1987) 30: 306–318. 3. J. Histochem. Cytochem. (1991) 39: 1,175–1,187. 4. J. Immunology (1998) 160: 3,812–3,819.|