Suitable for use in ELISA. Other applications not tested.
ELISA: 1:100-1:500 Chondroitinase ABC digestion of the target sample is required for antibody activity. Papain or pronase digestion of the target significantly reduces antibody activity.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
| Not Determined|
|Chondroitinase ABC-digested adult human aggrecan|
|Supplied as a liquid. No preservatives added.|
|Recognizes human Chondroitin-6-sulfate, recognizing the chondroitin-6-sulfate stubs of all high density cartilage proteoglycans across all species tested. Also recognizes chondroitinase ABC digested cartilage proteoglycans. Does not react with native proteoglycans. Species Crossreactivity: bovine, canine, rat, sheep, rabbit and mouse|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Poole, C. A. et al. (1991) Chondrons from articular cartilage. (IV). Immunolocalization of proteoglycan epitopes in isolated canine tibial chondrons. J. Histochem. Cytochem. 39: 1175-1187.|