Technical Data
C5067-70F
Chondroitin Sulfate Proteoglycan (NG2, CSPG)
Description:
NG2 is a high molecular weight, integral membrane chondroitin sulfate proteoglycan. It is found on the surfaces of an unusual class of glial cells within the developing and mature central nervous system that have the properties of oligodendrocyte precursor cells (i.e., O-2A progenitor cells). NG2 is also found on the surfaces of chondroblasts, proliferating capillary endothelial cells, some human melanoma cell lines, and on leukemic blasts in childhood acute lymphoblastic leukemia. The NG2 proteoglycan is likely to play a role in regulation of cell motility, axon outgrowth and the cellular responses to platelet-derived growth factor.

Applications:
Suitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry and ELISA. Other applications not tested.

Recommended Dilution:
Western blot: ~280kD. Blocking buffer: TBST, 5% dry milk.
Immunocytochemistry (Oligodendrocyte precursor cells): 1:200-1:500
Immunohistochemistry: Lightly fixed 4% paraformaldehyde for less than 2 hours, etc.. Blocking buffer: 2-4% normal serum. Antibody dilution buffer: PBS, 0.1% Triton X100.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG13H395Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceRatMouse
Concentration:
~1mg/ml
Immunogen:
Cell line expressing a truncated form of NG2. Species Sequence Homology: human and mouse
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes rat NG2 Chondroitin Sulfate Proteoglycan at ~280kD. Recognizes native, recombinant and protein from cells which express NG2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application reference: 1. Schonberg, D.L., et al., J. Neuropathology & Experimental Neurology 66: 1124-1135 (2007). General references: 1. Ponti, G., et al., PLoS ONE 3: e2366 (2008). 2. Svenningsen, A.F., et al., Neuon Glia Biol. 1: 85-93 (2004). 3. Fernandez, M., et al., PNAS USA 101(46): 16,363-16,368 (2004). 4. Barberi, T., et al., Nat. Neurosci. 21(10): 1200-1207 (2003).