Technical Data
C5067-70K
Chondroitin Sulfate Proteoglycan (NG2, CSPG)
Description:
The NG2 chondroitin sulfate proteoglycan is a valuable marker for many types of immature progenitor cells including pericytes/smooth muscle cells, chondroblasts and oligodendrocyte progenitors. NG2 is often expressed in melanomas, chondrosarcomas, glioblastomas and myeloid leukemias. Studies indicate that NG2 is involved in modulating cellular response to growth factors and cell motility; these are critical functions in proliferation and migration of immature progenitor cells and tumor cells. Oligodendrocyte precursor cells (OPCs) are a population of NG2 expressing cells in the brain that accumulate in response to CNS injuries. OPCs, together with microglia and astrocytes, form the glial scar, which is believed to inhibit or prevent axonal regeneration partly by producing growth inhibiting NG2 at the injury site8. It has been suggested that inhibiting NG2 at sites of spinal cord injury may increase beneficial axonal regeneration. Studies suggest that NG2 stimulates tumor growth and neovascularization in glioblastoma multiforme by inhibiting angiostatin and thus promoting angiogenesis. As NG2 is associated with increased tunmor cell proliferation, it may be a potential therapeutic marker in brain tumors.

Applications:
Suitable for use in Immunofluorescence, ELISA, Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications not tested.

Recommended Dilutions:
Immunofluorescence (Rat): 1-5ug/ml
ELISA (Human and Rat): 0.1-1ug/ml
Immunohistochemistry (Rat): Frozen sections
Immunoprecipitation (Rat): 10ug/reaction
Optimal dilutions to be determined by the researcher.

Source:
Ascites

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k4B4Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceRatMouse
Concentration:
~0.5mg/ml
Immunogen:
Purified rat NG2 protein.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes the membrane proximal epitope of human and rat NG2 protein at ~300kD. Reactivity has been confirmed with rat B49 glial cell line.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Stallcup WB, et al. J Neurocytol 31(6-7):423-35, 2002. 2. Stallcup WB, et al. J Cell Sci 114:2315-2325, 2001. 3. Grako KA and Stallcup WB. Exp Cell Res 221(1):231-40, 1995. 4. Grako KAJ, et al. Cell Sci 112(6):905-15, 1999. 5. Goretzki L, et al. J Biol Chem 274(24):16831-7, 1999. 6. Burg MA, et al. Exp Cell Res 235(1):254-64, 1997. 7. Fang X, et al. Mol Biol Cell (10):3373-87, 1999. 8. Chen ZJ, et al. J Neurocytol 31(6-7):481-95, 2002. 9. Jones LL, et al. J Neurosci 22(7):2792-2803, 2002. 10. Chekenya M, et al. FASEB J 16:586-588, 2002. 11. Chekenya M, et al. J Neurocytol (6-7):507-21, 2002.