Technical Data
C5069-94C
Chromatin Assembly Factor 1 (CAF1, CAF-1, CAF, Chromatin Assembly Factor 1 Subunit A, CAF-1 Subunit A, CHAF1A, CAF1B, Chromatin Assembly Factor I p150 Subunit, CAF-I 150kD Subunit, CAF1P150, CAF-I p150, hp150, MGC71229, P150)
Description:
Chromatin assembly factor 1 (CAF-1) is a histone H3/H4 chaperone complex that functions in de novo assembly of nucleosomes during DNA replication and nucleotide excision repair (1). Nucleosome assembly is a two-step process, involving initial deposition of a histone H3/H4 tetramer onto DNA, followed by the deposition of a pair of histone H2A/H2B dimers (1). CAF-1 interacts with PCNA and localizes to DNA replication and DNA repair foci, where it functions to assemble newly synthesized histone H3/H4 tetramers onto replicating DNA (2-6). Assembly
of histone H2A/H2B dimers requires additional assembly factors. The CAF-1 complex consists of three proteins: CHAF1A (p150), CHAF1B (p60) and RBAP48 (p48 or RBBP4). CHAF1A and CHAF1B proteins are specific for the CAF-1 complex, while RBAP48 is a component of multiple
chromatin modifying complexes (1). CHAF1A and CHAF1B expression levels correlate with cellular proliferation and both proteins are significantly down-regulated in quiescent cells (7).

Applications:
Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Immunofluorescence (IF-IC): 1:200
Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4C with gentle shaking, overnight.
Immunoprecipitation: 1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG11C1020Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to residues surrounding Thr293 of human CHAF1A.
Purity:
Purified
Form
Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, less than 0.02% sodium azide, 50% glycerol.
Specificity:
Recognizes endogenous levels of total CHAF1A. Species Crossreactivity: monkey.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
(1) Adams, C.R. and Kamakaka, R.T. (1999) Curr Opin Genet Dev 9, 185-90. (2) Smith, S. and Stillman, B. (1989) Cell 58, 15-25. (3) Smith, S. and Stillman, B. (1991) EMBO J 10, 971-80. (4) Shibahara, K. and Stillman, B. (1999) Cell 96, 575-85. (5) Moggs, J.G. et al. (2000) Mol Cell Biol 20, 1206-18. (6) Gaillard, P.H. et al. (1996) Cell 86, 887-96. (7) Polo, S.E. et al. (2004) Cancer Res 64, 2371-81.