		Technical Data
C5069-94C	Chromatin Assembly Factor 1 (CAF1, CAF-1, CAF, Chromatin Assembly Factor 1 Subunit A, CAF-1 Subunit A, CHAF1A, CAF1B, Chromatin Assembly Factor I p150 Subunit, CAF-I 150kD Subunit, CAF1P150, CAF-I p150, hp150, MGC71229, P150)

Description:
Chromatin assembly factor 1 (CAF-1) is a histone H3/H4 chaperone complex that functions in de novo assembly of nucleosomes during DNA replication and nucleotide excision repair (1). Nucleosome assembly is a two-step process, involving initial deposition of a histone H3/H4 tetramer onto DNA, followed by the deposition of a pair of histone H2A/H2B dimers (1). CAF-1 interacts with PCNA and localizes to DNA replication and DNA repair foci, where it functions to assemble newly synthesized histone H3/H4 tetramers onto replicating DNA (2-6). Assembly
of histone H2A/H2B dimers requires additional assembly factors. The CAF-1 complex consists of three proteins: CHAF1A (p150), CHAF1B (p60) and RBAP48 (p48 or RBBP4). CHAF1A and CHAF1B proteins are specific for the CAF-1 complex, while RBAP48 is a component of multiple
chromatin modifying complexes (1). CHAF1A and CHAF1B expression levels correlate with cellular proliferation and both proteins are significantly down-regulated in quiescent cells (7).

Applications:
Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Immunofluorescence (IF-IC): 1:200
Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Immunoprecipitation: 1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG	11C1020	Purified

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

Not determined

Immunogen:

Synthetic peptide corresponding to residues surrounding Thr293 of human CHAF1A.

Purity:

Purified

Form

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, less than 0.02% sodium azide, 50% glycerol.

Specificity:

Recognizes endogenous levels of total CHAF1A. Species Crossreactivity: monkey.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

(1) Adams, C.R. and Kamakaka, R.T. (1999) Curr Opin Genet Dev 9, 185-90. (2) Smith, S. and Stillman, B. (1989) Cell 58, 15-25. (3) Smith, S. and Stillman, B. (1991) EMBO J 10, 971-80. (4) Shibahara, K. and Stillman, B. (1999) Cell 96, 575-85. (5) Moggs, J.G. et al. (2000) Mol Cell Biol 20, 1206-18. (6) Gaillard, P.H. et al. (1996) Cell 86, 887-96. (7) Polo, S.E. et al. (2004) Cancer Res 64, 2371-81.