		Technical Data
C5069-94U	Chromatin Assembly Factor 1 Subunit A p150 (CHAF1A, CAF1 150kD Subunit, CAF1 p150 Subunit, CAF1 Subunit A, CAF1A, CAF1B, CAF-1 150kD Subunit, CAF-1 Subunit A, Chromatin Assembly Factor I 150kD, Chromatin Assembly Factor I p150 Subunit, CAF I 150kD Subunit

Description:
Chromatin assembly factor I (CAF1) is a nuclear complex consisting of p50, p60 (CHAF1B; MIM 601245), and p150 (CHAF1A) subunits that assembles histone octamers onto replicating DNA in vitro (Kaufman et al., 1995 [PubMed 7600578]).

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1000
ELISA: 1ug/ml-3ng/ml
Optimal dilutions to be determined by the researcher.

Hybridoma:
Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG2b	10B2214	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		4°C/-20°C (No Glycerol)	Blue Ice	Human	Mouse

Concentration:

~0.5mg/ml

Immunogen:

Recombinant protein corresponding to a portion of amino acids within aa21-120 of human CHAF1A.

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2. No preservative added (Azide free).

Specificity:

Recognizes human CHAF1A.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.