Technical Data
C5069-94U
Chromatin Assembly Factor 1 Subunit A p150 (CHAF1A, CAF1 150kD Subunit, CAF1 p150 Subunit, CAF1 Subunit A, CAF1A, CAF1B, CAF-1 150kD Subunit, CAF-1 Subunit A, Chromatin Assembly Factor I 150kD, Chromatin Assembly Factor I p150 Subunit, CAF I 150kD Subunit
Description:
Chromatin assembly factor I (CAF1) is a nuclear complex consisting of p50, p60 (CHAF1B; MIM 601245), and p150 (CHAF1A) subunits that assembles histone octamers onto replicating DNA in vitro (Kaufman et al., 1995 [PubMed 7600578]).

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1000
ELISA: 1ug/ml-3ng/ml
Optimal dilutions to be determined by the researcher.

Hybridoma:
Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b10B2214Affinity Purified
SizeStorageShippingSourceHost
100ul4C/-20C (No Glycerol)Blue IceHumanMouse
Concentration:
As reported
Immunogen:
Recombinant protein corresponding to a portion of amino acids within aa21-120 of human CHAF1A.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added (Azide free).
Specificity:
Recognizes human CHAF1A.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.