Technical Data
C5069-96
Chromogranin A (CGA, CHG A, Pancreastatin, Parathyroid secretory protein 1, Pituitary secretory protein I, Vasostatin, SPI)
Description:
Chromogranin A is a protein of 439aa which is encoded on chromosome 14. It is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheo-chromocytomas, paragangliomas, and other neuro-endocrine tumors. Coexpression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms.

Applications:
Suitable for use in Westrn Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Frozen and paraffin sections): 1:10
No proteolytic pretreatment.
Western Blot: 1:10
Immunofluorescence: 1:10
Optimal dilutions to be determined by the researcher.

Positive Control:
Pancreas and adrenal medulla

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k5F239Ascites
SizeStorageShippingSourceHost
1ml-20CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Isolated from human pheochromocytoma.
Purity:
Ascites
Form
Supplied as a liquid, 0.1% sodium azide. Dilute with TBS before use.
Specificity:
Recognizes human Chromogranin A. Represents an excellent marker for most neuroendocrine, neuronal and adrenal tumors: e.g. pheochromocytoma, paraganglioma, neuroblastoma, pancreatic islet cell carcinoma, gastroadenomas from pituitary and parathyroid gland. Weak reaction with Merkel cell tumors of skin and small cell lung carcinoma. Reacts with the 68kD protein (chromogranin A). The antigen is localized in secretory granules and, therefore, poorly granulated cells react weakly. Species Crossreactivity: monkey, rat, rabbit and porcine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Lloyd, RV et al. Specific endocrine tissue marker defined by a monoclonal antibody. Science 222:628-630 (1983)