		Technical Data
C5069-96B	Chromogranin A (CGA, CHG A, Pancreastatin, Parathyroid secretory protein 1, Pituitary secretory protein I, Vasostatin, SPI)

Description:
Chromogranin A (439aa) is encoded on chromosome 14. It is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheo-chromocytomas, paragangliomas, and other neuro-endocrine tumors. Coexpression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms.

Applications:
Suitable for Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
Immunohistochemistry:
Alcohol or formalin fixed deparaffinized tissue sections (ABC peroxidase): 1:500-1:2000
Methacarn-fixed deparaffinized tissue: 1:10,000

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1	2Q32	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		4°C (-20°C Glycerol)	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Chromogranin A

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide.

Specificity:

Specific for human Chromogranin A.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Riddell, K., et al., Lab. Invest. 56: 64A (1987). 2. Virchows Arch. A. Pathol. Anat. Histopathol. 413: 387-398 (1988). 3. Vet. Pathol. 26: 488–498 (1989). 4. Am. J. Surg. Pathol. 14: 703-713 (1990).