Technical Data
C5069-96E
Chromogranin A (CGA, CHG A, Pancreastatin, Parathyroid secretory protein 1, Pituitary secretory protein I, Vasostatin, SPI)
Description:
The chromogranins (A, B, and C) are major components of dense core granules of neuroendocrine cells and sympathetic nerves. They constitute a widely distributed family of acidic soluble proteins which range in molecular weight from 76-120kD. Each polypeptide subunit shares the some sequence homology. Polyclonal chromogranin A antibody is useful in the identification of neuroepthelial and neuroendocrine differentiation in neoplastic cells. It may be used with other neuroendocrine markers such as NSE, MOC-32, CD56, and synaptophysin to improve the tumor diagnosis.

Applications:
Suitable for use in Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry: Frozen and formalin-fixed, paraffin-embedded tissue sections using light microscopy
Optimal dilutions to be determined by the researcher.

Positive Control:
Human pancreas or neuroendocrine tumor that has been previously shown to express Chromogranin A

Epitope Retrieval: Not Required

Enzyme Digestion: Not Required

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
1ml-20CBlue IceHumanRabbit
Concentration:
~0.05mg/ml
Immunogen:
Purified Chromogranin A from human tissue with carcinoid syndrome. Expected Staining Pattern: Cytoplasmic.
Purity:
Purified
Form
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.1% sodium azide.
Specificity:
Recognizes human Chromogranin A fragments located at the C-terminal half, but not with fragments located at the N terminal half of the polypeptide. Recacts with Chromogranin A, which is present in the secretory vesicles of neuroendocrine cells such as in pancreas, adrenal medulla, pituitary, and thyroid.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.