Technical Data
C5069-96Q
Chromogranin A (CgA, CHGA, Parathyroid Secretory Protein 1, Pituitary Secretory Protein I, SP-I)
Description:
Chromogranin A is a precursor for the functional peptides pancreastatin, vasostatin, parastatin and catestatin, which act as negative modulators of hormone and neurotransmitter release in the neuroendocrine system. The measurement of elevated serum levels of chromogranin A is used diagnostically for detecting and monitoring carcinoid (neuroendocrine) tumors, and correlates with a poor prognosis in patients with prostate cancer.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:4,000
Western Blot: 0.1-0.3ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug4C/-20C (No Glycerol)Blue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Peptide sequence C-QGAKERAHQQKKH from the internal region of chromogranin A (NP_001266.1).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris-saline, pH 7.3, 0.02% sodium azide, 0.5% BSA.
Specificity:
Recognizes human chromogranin A.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Nobels, F.R.E. et al. (1997) Chromogranin A as serum marker for neuroendocrine neoplasia: comparison with neuron-specific enolase and the alpha-subunit of glycoprotein hormones. J. Clin. Endocrinol. Metab. 82: 2622-2628.