		Technical Data
C5069-96T	Chromogranin A (CgA, CHGA, Parathyroid Secretory Protein 1, Pituitary Secretory Protein I, SP-I)

Description:
This monoclonal antibody is a general marker for neurohormonal peptide producing cells (APUD) and neoplasms derived from these cells. Utrastructural studies have shown that the antigen is located in the secretory granules and therefore poorly granulated endocrine cells stain weakly or not at all. This should be considered when using this antibody.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Acetone fixed frozen sections, formalin fixed paraffin embedded tissue): 1:10 using the Indirect method. Pretreatment is not necessary. Dilute immediately before use with PBS or Tris.
Optimal dilutions to be determined by the researcher.

Positive Control:
Adrenal medulla, pancreas.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	LK2H10	Ascites

Size		Storage	Shipping	Source	Host
1ml		-20°C	Blue Ice	Human	Mouse

Concentration:

Not determined

Immunogen:

Human Pheochromocytoma Cellular Localization: Cytoplasm

Purity:

Ascites

Form

Supplied as a liquid in TRIS/HCL, 0.09% sodium azide.

Specificity:

Recognizes human chromogranin A. Predominantly reacts with a 68kD protein. Species Crossreactivity: Monkey, porcine

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Lloyd, R.V., et al., 1983, Science. vol. 222, 628-630.