Technical Data
C5069-96T
Chromogranin A (CgA, CHGA, Parathyroid Secretory Protein 1, Pituitary Secretory Protein I, SP-I)
Description:
This monoclonal antibody is a general marker for neurohormonal peptide producing cells (APUD) and neoplasms derived from these cells. Utrastructural studies have shown that the antigen is located in the secretory granules and therefore poorly granulated endocrine cells stain weakly or not at all. This should be considered when using this antibody.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Acetone fixed frozen sections, formalin fixed paraffin embedded tissue): 1:10 using the Indirect method. Pretreatment is not necessary. Dilute immediately before use with PBS or Tris.
Optimal dilutions to be determined by the researcher.

Positive Control:
Adrenal medulla, pancreas.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1LK2H10Ascites
SizeStorageShippingSourceHost
1ml-20CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Human Pheochromocytoma Cellular Localization: Cytoplasm
Purity:
Ascites
Form
Supplied as a liquid in TRIS/HCL, 0.09% sodium azide.
Specificity:
Recognizes human chromogranin A. Predominantly reacts with a 68kD protein. Species Crossreactivity: Monkey, porcine
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Lloyd, R.V., et al., 1983, Science. vol. 222, 628-630.