Technical Data
C5069-97
Chromogranin A (CGA, CHG A, Pancreastatin, Parathyroid secretory protein 1, Pituitary secretory protein I, Vasostatin, SPI)
Description:
Chromogranin A, a protein of 439-amino acid which is encoded on chromosome 14, is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheo-chromocytomas, paragangliomas, and other neuro-endocrine tumors. Coexpression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms.

Applications:
Suitable for use in Immunohistocemistry and Western Blot. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (formalin/paraffin): 0.5-1ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Western Blot: 1-2ug/ml at RT for 2 hours.
Optimal dilutions to be determined by the researcher.

Positive control:
PC-12 cells. Pancreas

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k0.N.218Affinity Purified
SizeStorageShippingSourceHost
500ul4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~0.2mg/ml
Immunogen:
Human pheochromocytoma. Cellular Localization: Cytoplasmic MW of antigen: 68-75kD.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.1% sodium azide. Also available without BSA and azide. See C5069-97X.
Specificity:
Recognizes human Chromogranin A at 68-75kD. Species Crosseactivity: monkey, porcine, rat and rabbit.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Lloyd, R.V., et. al., Science 222: 628-30 (1983).