Technical Data
C5069-97D
Chromogranin A (CGA, CHG A, Pancreastatin, Parathyroid secretory protein 1, Pituitary secretory protein I, Vasostatin, SPI)
Description:
The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Other peptides, including chromostatin, beta-granin, WE-14 and GE-25, are also derived from the full-length protein. However, biological activities for these molecules have not been shown.

Applications:
Suitable for use in ELISA (peptide) and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA (peptide): 1:4000 (detection limit).
Western Blot: 0.1-0.3ug/ml. ~ 50kD band observed in human kidney lysates (calculated MW of 50.7kD according to NP_001266.1).
Optimal dilutions to be determined by the researcher.

Positive Control: Human Kidney lysate

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide, C-QGAKERAHQQKKH, representing the internal region of the human protein according to NP_001266.1
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris-saline, pH7.3, containing 0.5% BSA and 0.02% sodium azide
Specificity:
Recognizes human Chromogranin A
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Li L, Yuan YZ, Lu J, Xia L, Zhu Y, Zhang YP, Qiao MM. , Treatment of pancreatic carcinoma by adenoviral mediated gene transfer of vasostatin in mice. ,Gut. 2006 Feb;55(2):259-65. Epub 2005 Nov 15., PMID: 16287901