		Technical Data
C5069-97E1	Chromogranin A (CgA, CHGA, Parathyroid Secretory Protein 1, Pituitary Secretory Protein I, SP-I)

Description:
chromogranin A (CgA), a secretory glycoprotein and member of the chromogranin/secretogranin family, widely expressed by cells of the neuroendocrine system and related tumors, which is used in immunohistochemistry as a valuable diagnostic marker for pheochromocytomas, paragangliomas and carcinoid, pancreatic and prostate tumors. CgA constitutes one of the most abundant components of secretory granules, and is the precursor to several functional peptides including vasostatin and pancreastatin.

Applications:
Suitable for use in Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry (paraffin): 1:250-1:1000 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections using sodium citrate buffer pH 6.0.
Optimal dilutions to be determined by the researcher.

Immunohistochemistry Positive Control:
Normal Pancreas

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	PHE5	Affinity Purified

Size		Storage	Shipping	Source	Host
25ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Human pheochromocytoma tissue

Purity:

Purified by Protein G affinity chromatography.

Form

Suppplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

Specificity:

Recognizes human Chromogranin A. Species crossreactivity: rat

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.