Technical Data
C5069-97E1
Chromogranin A (CgA, CHGA, Parathyroid Secretory Protein 1, Pituitary Secretory Protein I, SP-I)
Description:
Chromogranin A (CgA) is a secretory glycoprotein and member of the chromogranin/secretogranin family It is widely expressed by cells of the neuroendocrine system and related tumors, which is used in immunohistochemistry as a valuable diagnostic marker for pheochromocytomas, paragangliomas and carcinoid, pancreatic and prostate tumors. CgA constitutes one of the most abundant components of secretory granules, and is the precursor to several functional peptides including vasostatin and pancreastatin.

Applications:
Suitable for use in Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry (paraffin): 1:250-1:1000 Requires antigen retrieval using heat treatment prior to staining of paraffin sections using sodium citrate buffer pH 6.0.
Optimal dilutions to be determined by the researcher.

Immunohistochemistry Positive Control:
Normal Pancreas

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1PHE5Affinity Purified
SizeStorageShippingSourceHost
25ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Human pheochromocytoma tissue
Purity:
Purified by Protein G affinity chromatography.
Form
Suppplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity:
Recognizes human Chromogranin A. Does not recognize rat samples.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.