Technical Data
C5069-97P
Chromogranin A, NT (CGA, CHG A, Pancreastatin, Parathyroid Secretory Protein 1, Pituitary Secretory Protein I, Vasostatin, SPI)
Description:
Chromogranin A (CgA) is an 86kDa protein that is the major member of the granin family of acidic secretory glycoproteins located in neuroendocrine cells. CgA is believed to play a role in targeting peptide hormones and neurotransmitters to granules of the regulated pathways and
inhibit hormone and neurotransmitter release (1). Also, the widespread distribution of CgA has made the measurement of circulating immunoreactive CgA a valuable tool in the diagnosis of neuroendocrine neoplasia, and CgA immunohistochemistry can help to identify the neuroendocrine nature of tumors. The N-terminal domain of CgA inhibits tumor necrosis factor (TNF ) induced gap formation in human umbilical venous endothelial cells (2).
CgA levels which reflect neuroendocrine differentiation of prostatic carcinoma may have a diagnostic, therapeutic and prognostic role in the management of prostate cancer patients (3).

Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:10,000
Immunohistochemistry: 1:100-1:250
Immunocytochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to residues in the N-term of human Chromogranin A.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 50 mM Tris-Glycine (pH 7.4), 0.15 M sodium chloride, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Specificity:
Recognizes human Chromogranin A. Species Crossreactivity: Mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kim T, et al. J Clin Invest 115:1711-1713, 2005. 2. Eskeland NL, J Clin Invest 98:148-156, 1996.
3. Taupenot L, et al. N. Engl. J. Med. 348:1134-1149, 2003. 4. Berruti A, et al. Cancer 88:2590-2597, 2000.