Technical Data
C5069-97X
Chromogranin A (BSA & Azide Free) (CGA, CHG A, Pancreastatin, Parathyroid secretory protein 1, Pituitary secretory protein I, Vasostatin, SPI)
Description:
Chromogranin A is a protein of 439aa which is encoded on chromosome 14. It is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheo-chromocytomas, paragangliomas, and other neuro-endocrine tumors. Coexpression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms.

Applications:
Suitable for use in Immunohistocemistry and Western Blot. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Formalin/paraffin): 1:800 for 20 minutes at RT. Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes.
Western Blot: 1-2ug/ml for 2 hours at RT
Optimal dilutions to be determined by the researcher.

Positive control: PC-12 cells, Pancreas

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k0.N.218Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Human pheochromocytoma. Cellular Localization: Cytoplasmic
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See #C5069-97.
Specificity:
Recognizes human Chromogranin A at 68-75kD. Species Crossreactivity: monkey, porcine, rat and rabbit.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Lloyd, R.V., et. al., Science 222: 628-30 (1983).