Technical Data
C5069-99X
Chromogranin A (BSA & Azide Free) (CGA, CHG A, Pancreastatin, Parathyroid secretory protein 1, Pituitary secretory protein I, Vasostatin, SPI)
Description:
Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuro-endocrine tumors. Coexpression of chromogranin A and neuron specific enolase
(NSE) is common in neuroendocrine neoplasms.

Applications:
Suitable for use in Immunofluorescence and Immunohistochemistry . Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry (formalin/paraffin): 0.5-1.0ug/ml for 30min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0.
Optimal dilutions to be determined by the researcher.

Positive Control:
Pancreas

Source:
Ascites

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG10.N.219Affinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Human pheochromocytoma. Cellular Localization: Cytoplasmic.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See C5069-99.
Specificity:
Recognizes Chromogranin A at 68-75kD. Species Crossreactivity: Human and monkey. Does not react with rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Riddell K, et. al. Lab Invest 56:64A, 1987.