Technical Data
C5070
Chymotrypsin
Description:
Chymotrypsin preferentially catalyzes the hydrolysis of peptide bonds involving L-isomers of tyrosine, phenylalanine and tryptophan. It also readily acts upon amides and esters of susceptible amino acids. Chymotrypsin catalyzes the hydrolysis of bonds of leucyl, methionyl, asparaginyl and glutamyl residues. Chymotrypsinogen A is extracted from pancreatic tissue as a zymogen. Pancreatic extract contains approximately equal amounts of two forms of the zymogen, chymotrypsin A and chymotrypsin B. Chymotrypsin A may be activated to alpha, pi, beta, or gamma chymotrypsin. The enzyme is inhibited by heavy metals, the natural trypsin inhibitors to various degrees, an inhibitor from potato, and organophosphorous compounds.


Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C. For long-term storage, aliquot and store at 4C. Do Not Freeze! Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Note: We do not recommended adding glycerol as a cryopreservative.
TypeIsotypeCloneGrade
MabIgG32Q2239Ascites
SizeStorageShippingSourceHost
100ul4C Do Not FreezeBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Purified human pancreatic chymotrypsin.
Purity:
Ascites fluid.
Form
Liquid. Contains no preservative.
Specificity:
Recognizes chymotrypsin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.