Technical Data
C5070-07
Chymotrypsin, Human Pancreas
Description:
Chymotrypsin preferentially catalyzes the hydrolysis of peptide bonds involving L-isomers of tyrosine, phenylalanine and tryptophan. It also readily acts upon amides and esters of susceptible amino acids. Chymotrypsin catalyzes the hydrolysis of bonds of leucyl, methionyl, asparaginyl and glutamyl residues. Chymotrypsinogen A is extracted from pancreatic tissue as a zymogen. Pancreatic extract contains approximately equal amounts of two forms of the zymogen, chymotrypsin A and chymotrypsin B. Chymotrypsin A may be activated to alpha, pi, beta, or gamma chymotrypsin. The enzyme is inhibited by heavy metals, the natural trypsin inhibitors to various degrees, an inhibitor from potato, and organophosphorous compounds.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA Titer: 40,000tests/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
1ml4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Human pancreatic chymotrypsin.
Purity:
Sreum
Form
Supplied as a liquid in PBS, 40% glycerol.
Specificity:
Recognizes chymotrypsin 100%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.