Technical Data
C5073
Ciliary Neurotropic Factor, Recombinant, Rat (CNTF)
5ug
25ug
Molecular Biology Storage: -20CShipping: RT
Ciliary Neutrophic Factor (CNTF) is a potent neural factor originally characterized as a survival factor for chick ciliary neurons in vitro. CNTF is also important for the survival of other neural cell types including primary sensory neurons, motor neurons, basal forebrain neurons and type 2 astrocytes. CNTF is highly conserved across species and exhibits cross-species bioactivity.

Recombinant rat CNTF is a 22.7kD protein containing 199aa residues, lacking a hydrophobic N-terminal signal for secretion.

Biological Activity:
Determined by its ability to stimulate proliferation of human TF-1 cells using an ED50 concentration range of 25-35ng/ml.

Amino Acid Sequence:
AFAEQTPLTL HRRDLCSRSI WLARKIRSDL TALMESYVKH QGLNKNINLD SVDGVPVAST DRWSEMTEAE RLQENLQAYR TFQGMLTKLL EDQRVHFTPT EGDFHQAIHT LMLQVSAFAY QLEELMVLLE QKIPENEADG MPATVGDGGL FEKKLWGLKV LQELSQWTVR SIHDLRVISS HQMGISALES HYGAKDKQM

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile dH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
22.7kD
Source: E. coli
Purity: ~98% by SDS-PAGE and HPLC. Endotoxin: 0.1ng/ug (1EU/ug)
Form: Supplied as a lyophilized powder.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.