Technical Data
C5073-05
Ciliary Neurotropic Factor (CNTF)
Description:
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA (Indirect): 0.5-2ug/ml. Use 100ul/well antibody solution. When used in conjunction with compatible secondary reagents, 0.2-0.4ng/well of recombinant hCNT can be detected.
ELISA (Sandwich): 0.5-2ug/ml. Use 100ul/well antibody solution. When used in conjunction with C5073-05A as a detection antibody, 0.2-0.4ng/well of recombinant hCNTF
can be detected.
Western Blot: 0.1-0.2ug/ml. When used in conjunction with compatible secondary reagents, the detection limit is 1.5-3ng/lane, under reducing or non-reducing conditions.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug4°C (-20°C Glycerol)Blue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
Highly purified (>98%), human recombinant Ciliary Neurotropic Factor (hCNTF)
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile 40-50% glycerol, ddH2O to a concentration of 0.1-1mg/ml.
Specificity:
Recognizes human Ciliary Neurotropic Factor (CNTF)
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.