Technical Data
C5073-05A
Ciliary Neurotropic Factor (CNTF) (Biotin)
Description:
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 0.15-0.30ug/ml to detect hCNTF by direct ELISA, 100ul/well. Detection limit of 0.2ng/well of recombinant hCNTF.

Western Blot: 0.1-0.2 ug/ml. Detection limit for recombinant hCNTF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Optimal dilutions to be determined by the researcher.

Reconstitution:
Reconstitute to a concentration of 50ug/ml with sterile PBS, 0.1% BSA.

Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Country of Origin: USA
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug4°C (-20°C Glycerol)Blue IceHumanRabbit
Concentration:
~0.05mg/ml (after reconstitution)
Immunogen:
Highly purified, recombinant Ciliary Neurotropic Factor (CNTF)
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from a sterile-filtered solution in PBS, pH 7.4. Labeled with Biotin.
Specificity:
Recognizes Ciliary Neurotropic Factor (CNTF).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.