Technical Data
C5073-06C
Ciliary Neurotrophic Factor Recombinant, Human (CNTF)
100ug
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Technical Data |
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C5073-06C |
Ciliary Neurotrophic Factor Recombinant, Human (CNTF) |
100ug |
| Growth Factors, Cytokines | Storage: -20°CShipping: Dry Ice |
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CNTF (Ciliary neurotrophic factor) is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. This protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. CNTF protein is structurally related to leukemia inhibitory factor (LIF), interleukin-6 (IL-6), interleukin-11 (IL-11) and oncostatin M (OSM). It is localized in the cell nucleus subsequent to receptor binding. Recombinant human CNTF, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Molecular Weight: 25kD (220 aa) Sequences: MGSSHHHHHH SSGLVPRGSH MAFTEHSPLT PHRRDLCSRS IWLARKIRSD LTALTESYVK HQGLNKNINL DSADGMPVAS TDQWSELTEA ERLQENLQAY RTFHVLLARL LEDQQVHFTP TEGDFHQAIH TLLLQVAAFA YQIEELMILL EYKIPRNEAD GMPINVGDGG LFEKKLWGLK VLQELSQWTV RSIHDLRFIS SHQTGIPARG SHYIANNKKM Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Molecular Weight: 25kD |
Source: Recombinant Human E coli Purity: 95% by SDS PAGE. Concentration: ~1mg/ml Form: Supplied as a liquid in 20mM Tris pH 8.0, 1mM DTT. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Sendther M., et al. (1994), J Neurobiol. 25(11):1436-53. Robert F., et al. (2007), J. Biol. Chem. 282(46): 33421-33434.
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