Technical Data
C5073-09D
Ciliary Neurotrophic Factor (CNTF)
Description:
Applications:
Suitable for use in ELISA, Neutralization and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA (Indirect, Sandwich): 0.5ug-2ug/ml Detects 0.2-0.4ng/well of recombinant rat CNTF (using 100ul/well antibody solution).
Neutralization: 0.02-0.05ug/ml Yields one-half maximal inhibition [ND50] of the biological activity of rat CNTF (0.15ng/ml).
Western Blot: 0.1-0.2ug/ml Detects recombinant rat CNTF at 1.5-3.0ng/lane, under either reducing or non-reducing conditions.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer

Country of Origin:
USA
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug4C (-20C Glycerol)Blue IceRatRabbit
Concentration:
~1mg/ml
Immunogen:
Highly pure (> 98%) recombinant rat CNTF.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with 40-50% glycerol, ddH2O to a concentration of 0.1-1mg/ml.
Specificity:
Recognizes rat CNTF.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.