Technical Data
C5073-09E
Ciliary Neurotrophic Factor (CNTF)
Description:
CNTF is a survival promoting factor for different types of neurons in vitro and in vivo. The essential structural features for the biological function of human CNTF were investigated by Thier, M. et al. They showed that deletion of 14 N-terminal and 18 C-terminal amino acids significantly increased bioactivity compared to wild-type CNTF. CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. SUBUNIT: Homodimer.

Cellular Localization:
Cytoplasm.

Tissue Specificity:
Nervous system. PHARMACEUTICAL: CNTF is being tested under the name Axokine by Regeneron Pharmaceuticals for treatment of human motor neuron diseases, such as amyotrophic lateral sclerosis (ALS). As it induces substantial weight loss, preferentially of fat as opposed to lean body mass, it is being used for obesity treatment. Belongs to the CNTF family.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceHuman Rabbit
Concentration:
~1mg/ml
Immunogen:
Recombinant human CNTF.
Purity:
Serum
Form
Supplied as a lyophilized powder. Reconstitute in 100ul of sterile water. Centrifuge to remove any insoluble material.
Specificity:
This antibody specifically detects CNTF shown by western blot. Species Crossreactivity: This antiserum to known to react with rat, mouse and human CNTF protein.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. LF Lin etal (1989) Science 246, 1023-5 ; 2. KA Stockli etal (1991) J Cell Biol 115, 447-59 ; 3. I Saggio etal (1995) Embo J 14, 3045-54 ; 4. DM Hermann etal (2001) Neurobiol Dis 8, 655-66 ; 5. M Thier etal (1995) J Neurosci Res 40, 826-35