Technical Data
C5073-14C
CNTFR (Ciliary Neurotrophic Factor Receptor Subunit alpha, CNTF Receptor Subunit alpha, CNTFR-alpha)
Description:
This gene encodes a hematopoeitin/interferon-class receptor belonging to the cytokine superfamily of receptors. The encoded gene product represents the CNTF-specific alpha subunit of a heterotrimer forming the CNTF receptor complex, which also includes LIFR and gp130. The receptor is attached to the membrane by a glycosyl-phosphatidylinositol linkage and contains an immunoglobulin-like C2-type domain and a fibronectin type-III domain. Signal transduction requires that CNTF bind first to this alpha component, which permits the recruitment of gp130 and LIFR beta to form the tripartite receptor complex. Signal transduction stimulates gene expression, cell survival or differentiation in a variety of neuronal cell types. Alternative splicing has been observed at this locus and two variants, both encoding the same protein, have been identified.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:16,000
Western Blot: 0.3-1ug/ml, observed in mouse brain lysates on ~38-40kD bands
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to CRSNTYPKGFY, from mouse CNTFR, at the internal region of the protein (NP_671693.1, NP_001833.1).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity:
Recognizes mouse CNTFR. Species sequence homology: Canine, human and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Yokota H, Yoshikawa M, Hirabayashi H, Nakase H, Uranishi R, Nishimura F, Sugie Y, Ishizaka S, Sakaki T.
Expression of ciliary neurotrophic factor (CNTF), CNTF receptor alpha (CNTFR-alpha) following experimental intracerebral hemorrhage in rats. Neurosci Lett. 2005 Apr 4;377(3):170-5. Epub 2005 Jan 4.