		Technical Data
C5073-23B	Cartilage Intermediate-Layer Protein 1 (NT) (CILP-1, CILP, Nucleotide pyrophosphohydrolase, HGNC:1980, HsT18872) (Biotin)

Description:
The CILP-1 (cartilage intermediate-layer protein 1) gene product is a 138kD monomeric glycoprotein that is found in both hyaline and fibrocartilage. It is a precursor for two secreted, proteolytically generated products, a 90kD N-terminal CILP-1, and a 62kD C-terminal NTPPHase-homolog. The N-terminus is suggested to serve as both a matrix structural protein, and an IGF-I/TGF-beta1 suppressor sequestration molecule. Human CILP-1 spans aa22-720 of the CILP-1 precursor. It contains one TSP-1 domain (aa149-201) and a C2-type Ig-like region (aa309-395). Over aa1-720 of the CILP-1 precursor, human CILP-1 shares 89% aa identity with mouse CILP-1, and 42% aa identity with human CILP-2.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.1-0.2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, sterile 40-50% glycerol, 0.1% BSA, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG

Size		Storage	Shipping	Source	Host
50ug		-20°C	Blue Ice	Human	Goat

Concentration:

Not determined

Immunogen:

NS0-derived recombinant human CILP-1 N-terminal peptide aa22-720.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a lyophilized powder from 0.2um filtered solution in PBS, BSA. Reconstitute with sterile ddH2O, sterile 40-50% glycerol, 0.1% BSA. Labeled with Biotin.

Specificity:

Recognizes human CILP-1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.