Technical Data
C5806-02
Citrulline
Description:
The amino acid Citrulline is required to detoxify the liver from ammonia, which is a waste product of the body from oxidation. Citrulline promotes energy and assists with the immune system. This unusual amino acid is formed in the urea cycle by the addition of carbon dioxide and ammonia to ornithine. It is then combined with aspartic acid to form arginosuccinic acid, which later is metabolized into the amino acid arginine.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000 detected Citrulline on 10ug of human cornified cell lysate. The human cornified cell lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with C5086-02. Proteins were visualized using an IgG (HRP) donkey anti-rabbit and a chemiluminescence detection system.
Immunohistochemistry: 1:300 detected Citrulline in human skin tissue.
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-46E: IgG, H&L (HRP) Pab DkxRb
I1904-46K: IgG, H&L, X-Adsorbed (HRP) Pab DkxRb

Positive Control:
Human cornified cell lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceRabbit
Concentration:
Not Determined
Immunogen:
Linear peptide containing Citrulline.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 0.05% sodium azide.
Specificity:
Recognizes multiple deiminated keratins in human cornified skin. Thought to react preferentially with citrulline residues in glycine rich contexts. Species Sequence Homology: all species.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hans-Georg Kopp, Andrea T Hooper, M Johan Broekman, Scott T Avecilla, Isabelle Petit, Min Luo, Till Milde, Carlos A Ramos, Fan Zhang, Tabitha Kopp, Paul Bornstein, David K Jin, Aaron J Marcus, Shahin Rafii (2006) The Journal of clinical investigation. 116:3277-91. 2. Ishida-Yamamoto, Akemi, et al. (2002) J. Invest. Dermatol., 118: 282-7 (2002). 3. Ishida-Yamamoto, A, et al. (2000) J. Invest. Dermatol., 114: 701-5 (2000). 4. Senshu, T, et al. (1999) J. Dermatol. Sci., 21: 113-26 (1999). 5. Senshu, T, et al. (1996) Biochem. Biophys. Res. Commun., 225: 712-9 (1996).