Technical Data
C5815
c-Jun
Description:
Applications:
Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:2000 detected c-Jun in RIPA lysates from human A431 cells, and previously in Jurkat cell lysate. A431 RIPA cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with C5815 (1:1000ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunoprecipitation: 5ul
Immunocytochemistry: 1:200
Optimal dilutions to be determined by the researcher.

Positive Control: A0001-50
Non-stimulated A431 cell lysate. Use 10-20ug per lane.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
200ul4°C (-20°C Glycerol)Blue IceChickenRabbit
Concentration:
Not determined
Immunogen:
Trp E fusion-protein containing the DNA binding domain of avian c-Jun.
Purity:
Neat serum
Form
Supplied as a liquid, 0.05% sodium azide.
Specificity:
Recognizes avian c-Jun at ~ 35kD. Species crossreactivity: human and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.