Technical Data
C5815-10
c-Jun, phosphorylated (Ser63)
Description:
c-Jun is a component of the transcription factor AP-1, which binds and activates transcription at TRE/AP-1 elements. The transcriptional activity of c- Jun is regulated by phosphorylation at Ser63 and Ser73 (1,2). Extracellular signals, including growth factors, transforming oncoproteins and UV irradiation, stimulate phosphorylation of c-Jun at Ser63/73 and activate c- Jun-dependent transcription. Mutation of Ser63/73 renders c-Jun nonresponsive to mitogenic and stress induced signaling pathways. The MAP kinase homologue SAPK/JNK binds to the N-terminal region of c- Jun and phosphorylates c-Jun at Ser63/73. In addition, the activity of SAPK/JNK is stimulated by the same signals that activate c-Jun (3,4).

Applications:
Suitable for use in ELISA, Flow Cytometry, Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4C with gentle shaking, overnight.
Immunoprecipitation: 1:50
Immunocytochemistry (ABC): 1:1000
Flow Cytometry: 1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add sterile glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic phosphopeptide corresponding to residues around Ser63 of human c-Jun (KLH coupled).
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity:
Detects endogenous levels of c-Jun only when phosphorylated at Ser63. Does not recognize the phosphorylated forms of JunD or JunB. Species Crossreactivity: Mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Binetruy, B. et al. (1991) Nature 351, 122127. 2. Smeal, T. et al. (1991) Nature 354, 494496. 3. Derijard, B. et al. (1994) Cell 76, 10251037. 4. Kyriakis, J. M. et al. (1994) Nature 369, 156160. 5. de Ruiter, N.D. et al. (2000) Mol. Cell. Biol. 20, 84808488. 6. Garcia, M. et al. (2002) J. Neurosci. 22, 21742184 7. Gonzalez, M.V. et al. (2000) J. Cell Biol. 150, 11991208. 8. Kleemann, R. et al. (2000) Nature 408, 211216. 9. Lee, S. A. et al. (2001) J. Biol. Chem. 276, 1178311790. 10. Li, B. et al. (2002) EMBO J. 21, 324333. 11. Linseman, D.A. et al. (2001) J. Biol. Chem. 276, 3912339131. 12. Zhang, S.Q. (2000) Immunity 12, 301311.