Technical Data
C5815-10C
c-Jun, phosphorylated (Ser63) ELISA, BioAssay™, Antibody Pair
1Kit
Kits and Assays Storage: -20°CShipping: Blue Ice
c-Jun is a component of the transcription factor AP-1, which binds and activates transcription at TRE/AP-1 elements. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73 (1,2). Extracellular signals including growth factors, transforming oncoproteins and UV irradiation stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun-dependent transcription. Mutation of Ser63/73 renders c-Jun nonresponsive to mitogenic and stress-induced signaling pathways. The MAP kinase homologue SAPK/JNK binds to the amino-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73. In addition, the activity of SAPK/JNK is stimulated by the same signals that activate c-Jun (3,4).

Capture and Detection antibodies (100X stocks) and a HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are provided for performing 4 x 96 well ELISAs. Phospho-c-Jun (Ser63) Capture Antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysates are added, followed by c-Jun Detection Antibody and HRP-conjugated secondary antibody. HRP substrate, TMB, is added for color development. The magnitude of the absorbance at 450nm is proportional to the quantity of phospho-c-jun (Ser63) protein.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Binetruy, B. et al. (1991) Nature 351, 122–127.
2. Smeal, T. et al. (1991) Nature 354, 494–496.
3. Derijard, B. et al. (1994) Cell 76, 1025–1037.
4. Kyriakis, J.M. et al. (1994) Nature 369, 156–160.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.