Technical Data
C5815-10G
c-Jun, Total, ELISA Kit (Antibodies Only)
1Kit
Kits and Assays Storage: 4°C/-20°CShipping: Blue Ice
The Total c-Jun ELISA Kit (Antibodies Only) is being offered as an alternative to our Total c-Jun Sandwich ELISA Kit (C5815-10H). Capture and Detection antibodies (100X stocks) and a HRP-Conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are provided for performing 4 x 96 well ELISAs. The c-Jun capture antibody in PBS is coated overnight in a 96 well microplate. After blocking, cell lysates are added, followed by c-Jun detection antibody and HRP-Conjugated secondary antibody. HRP substrate, TMB, is added for color development. The magnitude of the absorbance at 450 nm is proportional to the quantity of total c-Jun protein.

c-Jun is a member of the Jun Family containing c-Jun, JunB and JunD, and is a component of
the transcription factor AP-1 (activator protein-1). AP-1 is composed of dimers of Fos, Jun and ATF family members and binds to and activates transcription at TRE/AP-1 elements (Reviewed in 1). Extracellular signals including growth factors, chemokines and stress activate AP-1-dependent transcription. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73 through SAPK/JNK (reviewed in 2). Knock-out studies in mice have shown that c-Jun is essential for embryogenesis (3), and subsequent studies have demonstrated roles for c-Jun in various tissues and developmental processes including axon regeneration (4), liver regeneration (5) and T cell development (6). AP-1 regulated genes exert diverse biological functions including cell proliferation, differentiation, and apoptosis, as well as transformation, invasion and metastasis, depending on cell type and context (7-9 ). Other target genes regulate survival as well as hypoxia and angiogenesis (8,10). c-Jun vival has emerged as a promising therapeutic target for cancer, vascular remodeling, acute inflammation, as well as rheumatoid arthritis (11-13).

Species Crossreactivity
Human, Mouse and Rat

Kit Components
C5815-10G1: Total c-Jun Capture Antibody (100X), 1x400ul. Pink cap.
C5815-10G2: Total c-Jun Detection Antibody (100X), 1x400ul. Blue cap.
C5815-10G3: IgG-HRP Antibody (1000X), 1x40ul. Anti-mouse IgG conjugated to HRP. Yellow cap.

Materials required but not supplied
Phosphate Buffered Saline (PBS)
Phosphate Buffered Saline (PBS) with Tween-20
TMB Substrate
Stop Solution
Blocking Buffer-PBS containing 0.05% Tween-20 and 1% BSA
96-well microplates (Corning® 96 Well Clear Polystyrene High Bind Stripwell™ Microplates (#2592) or Corning® 96 Well EIA/RIA Easy Wash™ Clear Flat Bottom Polystyrene High Bind Microplates (#3369))
Microplate reader

Storage and Stability
Store C5815-10G1 and C5815-10G2 at 4°C. Store C5815-10G3 at -20°C. Kit is stable for up to 6 months.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Jochum, W W. et al. (2001) . Oncogene 20, 2401–12. 2. Davis, R.J. (2000) Cell 103, 239–52. 3. Hilberg, F F. et al. (1993) . Nature 365, 179–81. 4. Raivich, G. et al. (2004) Neuron 43, 57–67. 5. Behrens, A. et al. (2002) EMBO J 21, 1782–90. 6. Riera-Sans, L. and Behrens, A. (2007) J Immunol 178, 5690–700. 7. Leppä, S. and Bohmann, D. (1999) Oncogene 18, 6158–62. 8. Shaulian, E. and Karin, M. (2002) Nat Cell Biol 4, E131–6. 9. Weiss, C. and Bohmann, D. (2004) Cell Cycle 3, 111–3. 10. Karamouzis, M.V V. et al. (2007) . Mol Cancer Res 5,109–20. 11. Kim, S. and Iwao, H. (2003) J Pharmacol Sci 91, 177-81. 12. Weiss, C. and Bohmann, D. (2004) Cell Cycle 3, 111–3. 13. Dass, C.R. and Choong, P P.F .F. (2008) . Pharmazie 63, 411–4.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.