Technical Data
C5815-10L
c-Jun (jun oncogene, Activator protein 1, AP1, AP-1, c-Jun, p39, Proto-oncogene c-jun, Transcription Factor AP-1, V-jun Avian Sarcoma Virus 17 Oncogene Homolog)
Description:
c-Jun is a component of the transcription factor AP-1 that binds and activates transcription at TRE/AP-1 elements and appears to be a major downstream target of the SAPK/JNK signaling pathway. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73. Extracellular signals including growth factors, transforming oncoproteins and UV irradiation stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun dependent transcription. Mutation of Ser63/73 renders c-Jun nonresponsive to mitogenic and stress induced
signaling pathways. The MAP kinase homologue, SAPK/JNK, binds to the N-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73. In addition, the activity of SAPK/JNK is stimulated by the same signals that activate c-Jun.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.1ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
NIH/3T3 cell lysate

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Linear peptide corresponding to the internal domain of c-Jun (KLH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity:
Recognizes the internal domain of human c-Jun. Species Crossreactivity: mouse. Species sequence homology: Rat, Bovine, Chicken, Canine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.