Suitable for use in Western Blot. Other applications not tested.
Immunoblot Analysis: A 1:1000 dilution of this lot detected phosphorylated c-Jun in RIPA lysates from 3T3 cells stimulated with 10ug/ml anisomycin for 30 minutes.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
| Not determined|
|KLH-conjugated, synthetic peptide corresponding to aa85-101 (NGHITT[pT]P[pT]PTQFLCPK) of human c-Jun. The immunizing sequence is identical in mouse and rat.|
|Purified by immunoaffinity chromatography.|
|Supplied as a liquid in 0.2M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.|
|Recognizes phosphorylated c-Jun (Thr91/Thr93), Mr 41kD. An additional unknown protein was also detected, Mr 65kD. Species Crossreactivity: Human and mouse. Predicted to crossreact with avian, rat, rabbit, bovine and porcine based on sequence homology.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Weiss, C., et al., EMBO J. 22: 3686-3695, 2003.|