Technical Data
C5815-14
c-Jun, phosphorylated (Thr91, Thr93)
Description:
Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Immunoblot Analysis: A 1:1000 dilution of this lot detected phosphorylated c-Jun in RIPA lysates from 3T3 cells stimulated with 10ug/ml anisomycin for 30 minutes.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
KLH-conjugated, synthetic peptide corresponding to aa85-101 (NGHITT[pT]P[pT]PTQFLCPK) of human c-Jun. The immunizing sequence is identical in mouse and rat.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 0.2M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
Specificity:
Recognizes phosphorylated c-Jun (Thr91/Thr93), Mr 41kD. An additional unknown protein was also detected, Mr 65kD. Species Crossreactivity: Human and mouse. Predicted to crossreact with avian, rat, rabbit, bovine and porcine based on sequence homology.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Weiss, C., et al., EMBO J. 22: 3686-3695, 2003.