Technical Data
C5819-50C
CLAC-P, NC3 Region (Collagen-like Alzheimer Amyloid Plaque Component)
Description:
Antibodies against senile plaque (SP) amyloid obtained a clone 9D2, which labeled amyloid fibrils in SPs and reacted with approximately 50/100kD polypeptides in Alzheimer's disease (AD) brains. The 9D2 antigen was identified as CLAC (collagen-like Alzheimer amyloid plaque component), and its precursor as CLAC-P/collagen type XXV. Both secreted and membrane-tethered forms of CLAC-P/collagen type XXV specifically bound to fibrillized Abeta, implicating these proteins in beta-amyloidogenesis and neuronal degeneration in AD.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
A synthetic peptide mapping to residues 430-443 [DYNGNLHEALQRIT] of the NC3 region of human and mouse CLAC-P.
Purity:
Purified by immunoaffinity chromatography.
Form
As reported
Specificity:
This antibody detects full-length CLAC-P and the secreted form of CLAC-P protein. It does not react with full-length or secreted forms of COLXIII protein or the COLXXIII protein. Species Crossreactivity: This antibody works in human and is expected to react with mouse protein.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hashimoto,T. et. al. EMBO J. 21 (7), 1524-1534 (2002).