Technical Data
CLAC-P, NC2-2 Region (Collagen-like Alzheimer Amyloid Plaque Component)
Antibodies against senile plaque (SP) amyloid obtained a clone 9D2, which labeled amyloid fibrils in SPs and reacted with approximately 50/100kD polypeptides in Alzheimer's disease (AD) brains. The 9D2 antigen was identified as CLAC (collagen-like Alzheimer amyloid plaque component), and its precursor as CLAC-P/collagen type XXV. Both secreted and membrane-tethered forms of CLAC-P/collagen type XXV specifically bound to fibrillized Abeta, implicating these proteins in beta-amyloidogenesis and neuronal degeneration in AD.

Western Blot: Use at 1:500 dilution. However, the investigator should determine the optimal dilution for a specific application such as immunohistochemistry.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20C or colder. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceHumanRabbit
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Reacts with residues 155-169 [KGEQGDQGPRMVFPK] of the NC2-2 region of human and mouse CLAC-P. Detects bands of 50, 70 and 100kD in SPs of AD brain, but not in diffuse plaques of non-demented aged individuals, diffuse deposits in sub-cortical structures and cerebellum in AD nor in cerebral amyloid angiopathy.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hashimoto,T. et. al. EMBO J. 21 (7), 1524-1534 (2002).