Technical Data
C5835-05
Clathrin
Description:
Clathrin consists of three heavy chain (192kD) and three light chains (LCa or LCb, 25-29kD) and self-assembles into a polyhedral cytoskeletal element. Self-assembly facilitates clathrins central role in membrane sorting and selective transport within the cell.

Applications:
Suitable for Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Frozen): 1:10
Western Blot: 1:100-1:500, ECL detection.
Optimal dilutions to be determined by the researcher.

Positive Control:
HeLa, SV-40, and RVF-SMC cultured cell lines.

Source:
Ascites

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute by adding sterile 40-50% glycerol, ddH2O. Aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgM2Q2166Highly Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceBovineMouse
Concentration:
~0.1mg/ml
Immunogen:
Coated vesicles (clathrin) of bovine brain.
Purity:
Purified by ammonium sulfate precipitation followed by gel filtration.
Form
Supplied as a lyophilized powder in PBS, pH 7.2, 1mg/ml BSA, 0.1% sodium azide. Reconstitute with 1ml sterile 40-50% glycerol, dH2O.
Specificity:
Recognizes the clathrin heavy chain from a diverse range of species. In nervous tissue it crossreacts with another coated vesicle polypeptide of 190kD which is not present in isolated clathrin triskelions. In some epithelial cells of certain species the antibody may crossreact with a cytokeratin component. It detects coated vesicles from different organs and tissues (e.g. brain, ovary, mammary gland). Species Crossreactivity: Human, bovine, rat and amphibian.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Bruder, G. and Wiedenmann, B. Exp. Cell Res. 164, 44962 (1986)