Technical Data
C5835-07B
Clathrin, Heavy Chain
Description:
Cellular Localization: Cell Membrane and Cytoplasmic

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG18J1021Affinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceBovineMouse
Concentration:
~2mg/ml
Immunogen:
Full length native protein (purified) (Cow). Epitope: The epitope recognized by the antibody resides within amino acids 207-264 of bovine CHC.
Purity:
Purified by Protein A affinity chromatography.
Form
15mM Sodium Azide; 0.01M PBS. pH 7.4
Specificity:
Reacts specifically with the clathrin heavy chain (CHC17), and does not crossreact with the muscle isoform CHC22. It is reported to be non-reactive with native clathrin in immunocytochemistry, and immunoprecipitation (but some staining is seen in Rat1 cells, following 4% paraformaldehyde- 0.5% Triton X-100 fixation). Species Crossreactivity: Crossreacts with Human, Mouse, Rat and bovine. Not yet tested in other species.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Liu SH et al. A novel clathrin homolog that co-distributes with cytoskeletal components functions in the trans-Golgi network. EMBO J 20:272-84 (2001). Beattie EC et al. NGF signals through TrkA to increase clathrin at the plasma membrane and enhance clathrin-mediated membrane trafficking. J Neurosci 20:7325-33 (2000). Wilde A et al. EGF receptor signaling stimulates SRC kinase phosphorylation of clathrin, influencing clathrin redistribution and EGF uptake. Cell 96:677-87 (1999). Nathke IS et al. Folding and trimerization of clathrin subunits at the triskelion hub. Cell 68:899-910 (1992).