Cellular Localization: Cell Membrane and Cytoplasmic
Suitable for use in Western Blot. Other applications not tested.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|Full length native protein (purified) (Cow). Epitope: The epitope recognized by the antibody resides within amino acids 207-264 of bovine CHC.|
|Purified by Protein A affinity chromatography.|
|15mM Sodium Azide; 0.01M PBS. pH 7.4|
|Reacts specifically with the clathrin heavy chain (CHC17), and does not crossreact with the muscle isoform CHC22. It is reported to be non-reactive with native clathrin in immunocytochemistry, and immunoprecipitation (but some staining is seen in Rat1 cells, following 4% paraformaldehyde- 0.5% Triton X-100 fixation). Species Crossreactivity: Crossreacts with Human, Mouse, Rat and bovine. Not yet tested in other species.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
Liu SH et al. A novel clathrin homolog that co-distributes with cytoskeletal components functions in the trans-Golgi network. EMBO J 20:272-84 (2001). <11226177>Beattie EC et al. NGF signals through TrkA to increase clathrin at the plasma membrane and enhance clathrin-mediated membrane trafficking. J Neurosci 20:7325-33 (2000). <11007890>Wilde A et al. EGF receptor signaling stimulates SRC kinase phosphorylation of clathrin, influencing clathrin redistribution and EGF uptake. Cell 96:677-87 (1999). <10089883>Nathke IS et al. Folding and trimerization of clathrin subunits at the triskelion hub. Cell 68:899-910 (1992). <1547490>|