Cellular Localization: Cytoplasmic
Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
| Not determined|
|Purified human brain clathrin heavy chain. Epitope: Electron microscopy and proteolysis mapping demonstrate that binding occurs towards the central hub of the triskelion, N-terminal to the light chain binding regions.|
|Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.|
|Detects clathrin heavy chain. Species Crossreactivity: Crossreacts with Human, Mouse and bovine.N ot yet tested in other species.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
This product has been used in:Brodsky FM Clathrin structure characterized with monoclonal antibodies. I. Analysis of multiple antigenic sites. J Cell Biol 101:2047-54 (1985). <2415533> Chang MP et al. Adaptor self-aggregation, adaptor-receptor recognition and binding of alpha-adaptin subunits to the plasma membrane contribute to recruitment of adaptor (AP2) components of clathrin-coated pits. EMBO J 12:2169-80 (1993). <8491205>Nathke IS et al. Folding and trimerization of clathrin subunits at the triskelion hub. Cell 68:899-910 (1992). <1547490>|