Technical Data
C5835-07C
Clathrin
Description:
Cellular Localization: Cytoplasmic

Applications:
Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG18J1022Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Purified human brain clathrin heavy chain. Epitope: Electron microscopy and proteolysis mapping demonstrate that binding occurs towards the central hub of the triskelion, N-terminal to the light chain binding regions.
Purity:
Purified
Form
Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.
Specificity:
Detects clathrin heavy chain. Species Crossreactivity: Crossreacts with Human, Mouse and bovine.N ot yet tested in other species.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
This product has been used in:Brodsky FM Clathrin structure characterized with monoclonal antibodies. I. Analysis of multiple antigenic sites. J Cell Biol 101:2047-54 (1985). Chang MP et al. Adaptor self-aggregation, adaptor-receptor recognition and binding of alpha-adaptin subunits to the plasma membrane contribute to recruitment of adaptor (AP2) components of clathrin-coated pits. EMBO J 12:2169-80 (1993). Nathke IS et al. Folding and trimerization of clathrin subunits at the triskelion hub. Cell 68:899-910 (1992).