Technical Data
C5835-07D
Clathrin Heavy Chain (Clathrin Heavy Chain 1, CLTC Hc, CHC17, Clathrin Heavy Polypeptide, CLH17, CLH-17, CLTCL2, Hc, KIAA0034)
Description:
Applications:
Suitable for use in ELISA, Immunofluorescence, Immunoprecipitation, and Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Hybridoma:
Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgM10B2250Highly Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant mouse SSRP1 (amino acid residues 150-413)(1).
Purity:
Purified by Ion Exchange chromatography.
Form
Supplied as a liquid in Tris, pH 8.0, 0.09% sodium azide.
Specificity:
Recognizes mouse clathrin heavy chain (CHC), a ubiquitously expressed protein and major component of clathrin-coated vesicles and pits, which combines with clathrin light chains (CLC) to form clathrin triskelions (3CHC and 3CLC), which then self-assemble into a polyhedral coat. Species Crossreactivity: rat, human, bovine, porcine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Macurek, L. et al. (2003) Monoclonal antibody BF-06 against the heavy chain of clathrin. Folia. Biol. (Praha) 49: 238-240.