Technical Data
C5835-07Y
Clathrin, Heavy Chain
Description:
Clathrin-coated pits, intracellular transport vesicles involved in endocytosis and other membrane trafficking processes, are multi-component units. (1,2). Clathrin, the most abundant protein in these vesicles, is formed of a basic assembly unit called a triskelion composed of three clathrin heavy chains and three clathrin light chains. Together with other coat components such as AP2, epsin and EPS15, triskelia are targeted to appropriate membrane fusion sites, where subsequent invagination, cargo protein uptake and vesicle internalization occurs (3,4).

Applications:
Suitable for use in Western Blot, Immunofluorescence/ Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Immunofluorescence / Immunocytochemistry: 1:400
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to residues surrounding Pro1663 of human Clathrin conjugated to KLH. Species sequence homology: porcine
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity:
Recognizes endogenous levels of total human Clathrin protein at 190kD. Species Crossreactivity: mouse, rat, monkey, bovine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Rodriguez-Boulan, E. et al. (2005) Nat. Rev. Mol. Cell Biol. 6, 233247. 2. Mousavi, S.A. et al. (2004) Biochem. J. 377, 116. 3. Rappoport, J.Z. et al. (2004) Traffic 5, 327237. 4. Brett, T.J. and Traub, L.M. (2006) Curr. Opin. Cell Biol. 18, 395406.