Technical Data
C5835-08
Clathrin, Light Chain
Description:
Clathrin consists of three heavy chains (192kD) and three light chains (LCa or LCb, 25-29kD). It self-assembles into a polyhedral cytoskeletal element. Self-assembly facilitates clathrin’s central role in membrane sorting and selective transport within the cell.

Applications:
Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Immunoprecipitation (Denatured verified): 2ug/mg protein using Protein A.
Western Blot: 1-2ug/ml for 2hrs at RT
Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT. Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.

Positive Control:
Placenta

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2b,k3F133Affinity Purified
SizeStorageShippingSourceHost
500ul-20°CBlue IceBovineMouse
Concentration:
~0.2mg/ml
Immunogen:
Synthetic peptide derived from bovine clathrin light chain (aa23-44). Cellular Localization: Cell membrane and cytoplasm.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See C5835-08X.
Specificity:
Recognizes bovine LCa and LCb (25-29kD). Species Crossreactivity: human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Jackson, A.P., et al., Nature 326: 154-159 (1987). 2. Nathke, I.S., et al., Cell 68: 899-910 (1992).