Technical Data
Clathrin, Light Chain
Cellular Localization: Cell Membrane and Cytoplasmic

Suitable for use in AP, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG2b8J1024Affinity Purified
100ul-20CBlue IceBovineMouse
Not determined
Synthetic peptide, corresponding to amino acids 23-44 of Cow Clathrin Light Chain.
Purified by immunoaffinity chromatography.
0.01M PBS. pH 7.4
Reacts specifically with the clathrin light chain. The epitope recognized by the antibody resides within amino acids 23-44 of bovine clathrin light chains, LCa and LCb. This region is not involved in heavy chain binding, and is completely conserved in all mammals. Species Crossreactivity: Crossreacts with Human, Mouse, Rat, bovine and S. cerevisiae. Not yet tested in other species.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Liu SH et al. A novel clathrin homolog that co-distributes with cytoskeletal components functions in the trans-Golgi network. EMBO J 20:272-84 (2001). Ybe JA et al. Clathrin self-assembly is regulated by three light-chain residues controlling the formation of critical salt bridges. EMBO J 17:1297-303 (1998). Liu SH et al. Regulation of clathrin assembly and trimerization defined using recombinant triskelion hubs. Cell 83:257-67 (1995). Nathke IS et al. Folding and trimerization of clathrin subunits at the triskelion hub. Cell 68:899-910 (1992).