Cellular Localization: Cell Membrane and Cytoplasmic
Suitable for use in AP, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
| Not determined|
|Synthetic peptide, corresponding to amino acids 23-44 of Cow Clathrin Light Chain.|
|Purified by immunoaffinity chromatography.|
|0.01M PBS. pH 7.4|
|Reacts specifically with the clathrin light chain. The epitope recognized by the antibody resides within amino acids 23-44 of bovine clathrin light chains, LCa and LCb. This region is not involved in heavy chain binding, and is completely conserved in all mammals. Species Crossreactivity: Crossreacts with Human, Mouse, Rat, bovine and S. cerevisiae. Not yet tested in other species.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
Liu SH et al. A novel clathrin homolog that co-distributes with cytoskeletal components functions in the trans-Golgi network. EMBO J 20:272-84 (2001). <11226177>Ybe JA et al. Clathrin self-assembly is regulated by three light-chain residues controlling the formation of critical salt bridges. EMBO J 17:1297-303 (1998). <9482727>Liu SH et al. Regulation of clathrin assembly and trimerization defined using recombinant triskelion hubs. Cell 83:257-67 (1995). <7585943>Nathke IS et al. Folding and trimerization of clathrin subunits at the triskelion hub. Cell 68:899-910 (1992). <1547490>|