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Technical Data |
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C5835-08C |
Clathrin Light Chain (Clathrin Light Chain A, Clathrin Light Chain LCA, CLTA, Clathrin Light Chain B, Clathrin Light Chain LCB, CLTB, Clathrin Light Polypeptide, Clathrin Light Polypeptide A, Clathrin Light Polypeptide LCA, LCA, Clathrin Light Polypeptide |
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Description: Stains coated vesicles from various organs and tissues tested e.g. brain, mammary gland, ovaries. It also reacts with the cell lines, HeLa, SV-40 and RVF-SMC.Clathrin is a protein which assembles into a polyhedral network on the cell membrane as the membrane invaginates, forming a coated pit which is essential to endocytosis. It is composed of three polypeptides, a 180 kDa heavy chain and two 32-38 kDa light chains which combine to create a distinct three-legged triskelion. It is this morphology which allows clathrin to form its unique polyhedral network.In nervous tissue. Cross-reacts with another coated vesicle protein of 190 kDa which is not present in isolated clathrin triskelions. In some epithelial cells of certain species, this antibody may react with a cytokeratin component. Applications: Suitable for use in Immunohistochemistry (frozen) and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, dH2O. Aliquot and store at -20°C. Reconstituted product is stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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