Technical Data
C5835-08E
CLTA (Clathrin Light Chain A, Lca)
Description:
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:32,000
Western Blot: 0.1-0.3ug/ml, observed in human brain (frontal cortex) lysates on ~35, 22-23kD bands
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to CDFNPKSSKQAKD, from human CTLA, at the internal region of the protein (NP_001824.1, NP_009027.1, NP_001070145.1, NP_001825.1, NP_009028.1).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity:
Recognizes human CTLA. Species sequence homology: Bovine, canine, mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Poupon V, Girard M, Legendre-Guillemin V, Thomas S, Bourbonniere L, Philie J, Bright NA, McPherson PS. Clathrin light chains function in mannose phosphate receptor trafficking via regulation of actin assembly. Proc Natl Acad Sci U S A. 2008 Jan 8;105(1):168-73. Epub 2007 Dec 28.