		Technical Data
C5835-08E	CLTA (Clathrin Light Chain A, Lca)

Description:
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:32,000
Western Blot: 0.1-0.3ug/ml, observed in human brain (frontal cortex) lysates on ~35, 22-23kD bands
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab			Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Goat

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide corresponding to CDFNPKSSKQAKD, from human CTLA, at the internal region of the protein (NP_001824.1, NP_009027.1, NP_001070145.1, NP_001825.1, NP_009028.1).

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

Specificity:

Recognizes human CTLA. Species sequence homology: Bovine, canine, mouse and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Poupon V, Girard M, Legendre-Guillemin V, Thomas S, Bourbonniere L, Philie J, Bright NA, McPherson PS. Clathrin light chains function in mannose phosphate receptor trafficking via regulation of actin assembly. Proc Natl Acad Sci U S A. 2008 Jan 8;105(1):168-73. Epub 2007 Dec 28.