Technical Data
C5835-08X
Clathrin, Light Chain (BSA & Azide Free)
Description:
Clathrin consists of three heavy chain (192kD) and three light chains (LCa or LCb, 25-29kD). It self-assembles into a polyhedral cytoskeletal element. Self-assembly facilitates clathrin’s central role in membrane sorting and selective transport within the cell.

Applications:
Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Immunoprecipitation (Denatured verified): 2ug/mg protein using Protein A.
Western Blot: 1-2ug/ml for 2hrs at RT
Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 min at RT. Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.

Positive Control: Placenta

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

TypeIsotypeCloneGrade
MabIgG2b,k3F133Affinity Purified
SizeStorageShippingSourceHost
100ul4°C (-20°C Glycerol)Blue IceBovineMouse
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide derived from bovine clathrin light chain corresponding to the conserved region of the light chains. MW of Antigen: 25-29kD Epitope: aa23-44 Cellular Localization: Cell membrane and cytoplasm
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See C5835-08.
Specificity:
Recognizes both bovine LCa and LCb. Species Crossreactivity: human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Jackson AP, et al. 1987. Nature. 326:154-159. 2. Nathke IS, et al. 1992. Cell. 68:899-910.