Technical Data
Clathrin, Light Chain (BSA & Azide Free)
Clathrin consists of three heavy chain (192kD) and three light chains (LCa or LCb, 25-29kD). It self-assembles into a polyhedral cytoskeletal element. Self-assembly facilitates clathrin’s central role in membrane sorting and selective transport within the cell.

Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Immunoprecipitation (Denatured verified): 2ug/mg protein using Protein A.
Western Blot: 1-2ug/ml for 2hrs at RT
Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 min at RT. Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.

Positive Control: Placenta

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

MabIgG2b,k3F133Affinity Purified
100ul4°C (-20°C Glycerol)Blue IceBovineMouse
Synthetic peptide derived from bovine clathrin light chain corresponding to the conserved region of the light chains. MW of Antigen: 25-29kD Epitope: aa23-44 Cellular Localization: Cell membrane and cytoplasm
Purified by Protein A affinity chromatography.
Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See C5835-08.
Recognizes both bovine LCa and LCb. Species Crossreactivity: human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Jackson AP, et al. 1987. Nature. 326:154-159. 2. Nathke IS, et al. 1992. Cell. 68:899-910.