Technical Data
C5835-08X
Clathrin, Light Chain (BSA & Azide Free)
|
Technical Data |
|
C5835-08X |
Clathrin, Light Chain (BSA & Azide Free) |
|
|
Description: Clathrin consists of three heavy chain (192kD) and three light chains (LCa or LCb, 25-29kD). It self-assembles into a polyhedral cytoskeletal element. Self-assembly facilitates clathrin’s central role in membrane sorting and selective transport within the cell. Applications: Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Immunoprecipitation (Denatured verified): 2ug/mg protein using Protein A. Western Blot: 1-2ug/ml for 2hrs at RT Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 min at RT. Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Optimal dilutions to be determined by the researcher. Positive Control: Placenta Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
|
|||||||||||
|
||||||||||||
|
||||||||||||
|
||||||||||||