Technical Data
C5835-09A
Clathrin, Light Chain, Isoform a
Description:
Clathrin is the main structural component of the lattice covering the cytoplasmic face of the coated pits and coated vesicles in which specific macromolecules are entrapped in the process of receptor-mediated endocytosis. Clathrin is a large, soluble protein composed of heavy chains and light chains. Two genes encode the clathrin light chain proteins. The light chains are believed to be regulatory elements in clathrin function. The brain-specific light chain variant contains an insert encoding 18aa immediately downstream of the region encoding the heavy chain binding domain. Two transcript variants encoding different isoforms have been found for this gene.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgYAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanChicken
Concentration:
As reported
Immunogen:
Synthetic peptide corresponding to aa1-211
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity:
Recognizes human Clathrin, Light Chain, isoform a.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.