Technical Data
Claudin 1 (CLDN1)
Tight junctions are specialized regions of cell-cell contact that are particularly abundant in luminal epithelial cell sheets. TJ strands on adjacent cells are presumed to interact with each other to form a “molecular gasket” that prevents ions, water and other molecules from leaking between cells and thus, from one side of the sheet to the other. The 20 known claudin proteins are major structural components of tight junctions.
Immuno uorescence studies revealed that tight junctions contained Claudin-3, -4 and –8 in the liver and/or kidney and Claudin-1, -2, in the broblast. It is also reported that Claudins-2, -3, -4 and -5 showed heterogenic expression and subcellular localization in the rat liver, pancreas and gut. In normal tissues, most of the tissues stained positively. This staining pattern is cytoplasmic with the exception of normal skin, which showed a clear membrane staining. In the 20 cases of matched colon cancer, the staining pattern of matched mucosa, either adjacent or remoted part, was mainly cytoplasmic staining.

Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: Formalin-fixed paraffin-embedded tissue sections. Epitope Retrieval with EDTA, pH 8, is required. See protocol. Enzyme digestion is not required.
Optimal dilutions to be determined by the researcher.

Expected Staining Pattern:
Cytoplasmic with some membrane and nuclear

Positve Control Tissue:
Normal skin

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
500ul-20°CBlue IceHumanRabbit
Synthetic peptide corresponding to the C-terminus of the human Claudin 1.
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide.
Recognizes human Claudin 1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Morita, K., et al., Proc. Natl. Acad. Sci. USA 96: 511-516 (1999). 2. Furuse, M., et al., J. Cell. Biol. 143: 391-401 (1998). 3. Totsch, M., et al., Histopathology 26: 357-361 (1995).