Technical Data
C5838-02C
Claudin 1 (CLDN1)
Description:
Claudin 1-8 proteins are a family of transmembrane proteins associated with tight junctions. Tight junctions are specialized regions of cell to cell contact; made up of network of strands to act as a molecular gasket for preventing the leakage of ions, water etc. between cells. They are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Different tissues exhibit different Claudin composition.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 2-4ug/ml for 2 hours at RT
Immunohistochemistry (Formalin/paraffin): 1:50-1:100 for 20 minutes. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.

Positive Control:
T47D cells, Skin or Breast carcinoma.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
250ul-20CBlue IceHumanRabbit
Concentration:
~0.2mg/ml
Immunogen:
Synthetic peptide corresponding to the C-terminus of human Claudin 1. Cellular Localization: Cell membrane on skin and cytoplasmic and nuclear on breast carcinoma.
Purity:
Purified
Form
Supplied as a liquid in 10mM PBS, pH 7.6, 0.2% BSA, 15mM sodium azide.
Specificity:
Recognizes human Claudin 1 at 22kD. Species Crossreactivity: monkey, mouse, canine, and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.